Figure 3.

MED17 is required for sucrose-regulated root development in Arabidopsis. A–C, Root phenotype of Col-0 and med17 seedlings after treatment with sucrose. Four-day-old Col-0 and med17 seedlings were transferred to ½ MS plates containing different concentrations of sucrose (30 or 90 mM) for another 4 days. ImageJ was used to count lateral root (LR) number and measure the PR length. Data shown are average of three independent biological replicates containing at least 15 seedlings. Bar plots represent mean values, and error bars denote Se. Statistical difference has been depicted by P-value as assessed by one-way ANOVA and Tukey's HSD post hoc test. Length of the scale bar in the image is 5 mm. D, Confocal images of root tips showing EDU staining. Length of the scale bar in the image is 100 µm. E, Graphical representation of total number of EDU-stained nuclei. D, E, Four-day-old Col-0 and med17 seedlings were treated with 90 mM sucrose for 3 h followed by EDU staining and confocal imaging. Data shown are average of three independent biological replicates containing at least 10 seedlings. Bar plots represent mean values, and error bars denote Se. Statistical difference has been depicted by P-value as assessed by one-way ANOVA and Tukey's HSD post hoc test. For all the graphs, P-value of 0.05 or lower (P ≤ 0.05) was considered statistically significant, whereas P-value greater than 0.05 (P > 0.05) was considered non-significant (ns).