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. 2023 Feb 28;192(2):1548–1568. doi: 10.1093/plphys/kiad129

Figure 8.

Figure 8

MED17 interacts with E2FA and E2FB. A, BiFC assay showing interaction of MED17 with E2FA and E2FB. MED17 and E2FA/B were cloned into CD3-1648 and CD3-1651 vectors, respectively. Interactions were confirmed on the basis of YFP signals. No YFP signals were observed between YFP N MED17–C YFP, YFP N–C YFP E2FA, and YFP N–C YFP E2FB. Hence, these were used as negative controls. Length of the scale bar in the image is 50 µm. B, Pull-down assay showing interaction of MED17 with E2FA and E2FB. Whole protein extract of pgE2FA-GFP or pgE2FB-GFP seedling was incubated with purified GST-MED17 or GST alone. Immuno pull-down was performed using anti-GST antibody, and the GFP-tagged proteins remaining on the beads were detected by anti-GFP antibody. Only GST was used as a negative control. Data shown are the representative of two independent biological replicates (n = 2). For all the graphs, P-value of 0.05 or lower (P ≤ 0.05) was considered statistically significant, whereas P-value greater than 0.05 (P > 0.05) was considered non-significant (ns).