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. 2023 Feb 28;192(2):1000–1015. doi: 10.1093/plphys/kiad123

Figure 1.

Figure 1.

Phenotypes of erd1a and erd1b T-DNA mutants. Phenotypes of T-DNA insertion mutants in the ERD1AA), D) and ERD1BK) genes are shown along with corresponding diagrams of the gene exon-intron structures with the location of the T-DNA insertion sites B), M) and the RT-PCR analysis using oligos that span the insertion sites, showing disruption in gene expression C), L). The erd1a mutant phenotype was complemented by the pERD1A::ERD1A-GFP construct (A, right). In contrast to Col-0 E), G), I), stamens of erd1a flowers failed to release mature pollen F), H), and Alexander's staining showed that erd1a pollen was not viable J). For I) and J), scale bars represent 100 µm. N)ERD1A and ERD1B expression level in roots and shoots of 10-d-old plants was measured by RT-qPCR. Student's t-test, error bars, ± Sd, n = 4, ****P < 0.0001. O) Expression of the ERD1-GFP and ERD1B-GFP transgenes was analyzed by RT-qPCR with primers targeting GFP. Expression is shown relative to ACTIN2 (ACT2). Error bars, ± Sd, n ≥ 3. P) The erd1a mutant was complemented by expressing ERD1A via its native promoter (pERD1A::ERD1A-GFP) or the CaMV35S promoter (35S::ERD1A-GFP), or by expressing ERD1B-GFP with the CaMV35S promoter (35S::ERD1B-GFP). ERD1B-GFP expression via the ERD1A promoter (pERD1A::ERD1B-GFP) partially complemented the erd1a mutant (middle right). Four-week-old plants of 2 independent lines for each complementation are shown.