Table I.
Effect of low temperature on Al-induced malate efflux from root apexes of Al-resistant wheat (cv Atlas)
Treatment
|
Malate Efflux
|
||
---|---|---|---|
Pretreatment | Treatment | 25°C Grown | 4°C Grown |
nmol apex−1 30 min−1 | |||
− | −Al at 25°C | 0.05 ± 0.02 | 0.05 ± 0.01 |
− | +Al at 25°C | 0.90 ± 0.03 | 0.91 ± 0.02 |
− | +Al at 4°C | 0.04 ± 0.01 | 0.15 ± 0.01 |
+Al at 4°C | +Al at 25°C | 1.04 ± 0.02 | 0.90 ± 0.02 |
From seedlings grown at either 25°C or 4°C for 24 h before the treatment, root apexes were excised and exposed to 200 μm CaCl2 solution (Ca solution) or the Ca solution containing 200 μm Al (pH 4.2; Ca+Al solution) at 25°C or 4°C. Periods of pretreatment and treatment were 30 min each. Values are means ± se (n = 3).