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. 2023 Feb 24;192(2):967–981. doi: 10.1093/plphys/kiad117

Figure 1.

Figure 1.

Map-based cloning of GS10. Plant morphology and grain shape phenotype between GLA4 and CSSL50. Scale bar, 5 cm (A), 1 cm (B, both panels). C)GS10 was initially mapped between marker loci OP10-6 and OP10-7 using 155 BC2F2 plants based on the phenotype of GW and grain length/GW. D)GS10 was then fine-mapped to a 10-kilobase (kb) genomic region between the marker SNP10-6 and SNP10-3 using the BC2F4 population. Genotype (left), GW (middle), and grain length/GW (right) were shown for recombinant plants and the control plants. White and black bars represent chromosomal segments homozygous for GLA4 and W1943 alleles, respectively. The only 1 predicted open reading frame in the 10-kb delimited region among 3 CSSLs and their parents. The numbers below the molecular markers in (D) indicate the number of recombinants. Statistics of grain length E), GW F), grain length/GW G), grain thickness H), and grain weight I) between GLA4 and CSSSL50, n ≥ 18. Values are given as the mean ± Sd. Student's t-test significant difference, ***P < 0.001; ns, not significant.