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. 2023 Feb 24;192(2):982–999. doi: 10.1093/plphys/kiad121

Figure 1.

Figure 1.

Ascorbic acid (AsA) and AceGGP3 are induced by cold stress and alleviated ROS damage. A) The species being studied represent the major clades and subclades in the plant kingdom. B) AsA concentrations increased in most plants after exposure to cold (4 °C) for 0 h, 6 h, 12 h, and 2 d (n = 4–6). C) Relative AsA content of wild-type (WT), AceGGP3-overexpression (OE14 and OE20), and AceGGP3-edited (KO13 and KO15) lines of kiwifruit calli (AsA content of wild-type was taken as 5 for normalization). D) Under cold treatment (4 °C, 6 h), fewer fluoresce (ROS) were detected in AceGGP3-overexpression kiwifruit calli than that in AceGGP3-editing calli and WT. E–I) The content of O2.−E), H2O2F), proline G), MDA H), and electrolyte leakage (n = 4) I) in WT, AceGGP3 overexpression and editing calli after 25 °C (control) or 4 °C (cold stress) for 6 h. Error bars denote the standard deviation (± SD), n = 3 to 4. Significant differences were detected by t-test (*P < 0.05; **P < 0.01; ***P < 0.001).