Table 1.

Spironolactone did not affect (A) chow or (B) water intake in mice.

		Vehicle-treated				Spironolactone-treated
		Males		Females		Males		Females
A	Total chow** intake (g)	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
	6 h	0.95	0.172	1.28	0.235	0.43	0.197	1.22	0.397
	24 h####	2.51	0.343	4.00†	0.259	2.11	0.404	4.00†	0.886
B	Total water* intake (mL)	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
	6 h	0.80	0.096	1.06	0.225	0.80	0.177	0.98	0.328
	24 h####	1.69	0.164	3.16††	0.293	1.68	0.263	2.54	0.572

Male: n = 8; female: n = 5.

^*p < 0.05

^**p < 0.01, male vs. female (overall sex effect; three-way ANOVA).

^####p < 0.0001, 6 h vs. 24 h (overall time effect; three-way ANOVA).

^†p < 0.05, male vehicle-treated 24 h vs. female vehicle-treated 24 h and male spironolactone-treated 24 h vs. female-spironolactone treated 24 h

^††p < 0.01, male vehicle-treated 24 h vs. female vehicle-treated 24 h (sex vs. time interaction; three-way repeated measures ANOVA followed by Holm–Sidak post hoc test).