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The compositions of lysis and elution buffers (pH = 7) for protein purification.
| Reagents | Lysis Buffer A | Lysis Buffer B | Elution Buffer |
|---|---|---|---|
| NaH2PO4 | – | – | 20 mM |
| Tris | 24.22 g/L | 8.1 g/L | – |
| NaCl | – | – | 500 mM |
| Imidazole | – | – | 500 mM |
| EDTA | 0.2 g/L | 0.05 g/L | – |
| Sucrose | 170 g/L | 43 g/L | – |
