Fig. 4.

Glucose starvation is not detrimental to LCMV propagation. MRC-5 cells were LCMV-infected at an MOI of 3. After adsorption, the inoculum was removed and replaced with either complete or glucose-free medium (w/o glucose), followed by a 24-h incubation. A Twenty-four hours after medium exchange, cell viability was measured by LIVE/DEAD™ Fixable Dead Cell Stain Kit. Data from four independent experiments are shown as a scatter of individual values of geometric mean fluorescence intensity. Mean and SD are shown as column and error bars, respectively. B LCMV titer in the extracellular media was determined by a focus-forming assay. Data from six independent experiments are shown as a scatter of individual values. Mean and SD are shown as column and error bars, respectively. C Total RNA was isolated, and intracellular LCMV RNA was detected by amplification of the NP gene fragment using RT-qPCR. Copy numbers of viral NP were calculated using a standard curve. Data from three independent experiments are shown as a scatter of individual values. Mean and SD are shown as column and error bars, respectively. Statistical differences between groups were analyzed using unpaired t-test (C) or unpaired t-test with Welch’s correction (A, B). FFU/ml, focus-forming units per milliliter