Fig. 7.

Inhibitory effect of 2-DG on LCMV propagation is dependent on the time of its addition. A Schematic of the experimental design. B MRC-5 cells were infected with LCMV at an MOI of 3. After adsorption, the inoculum was removed and replaced with complete medium, to which 10 mM 2-DG was added at different time points after infection. Supernatants were collected 24 h after medium exchange and LCMV titers were determined by a focus-forming assay. Data from four independent experiments are shown as a scatter of individual values. Mean and SD are shown as column and error bars, respectively. Statistical differences between groups were analyzed using one-way ANOVA and Tukey’s post hoc test. Different letters above the columns indicate significant differences (P ≤ 0.05), while identical letters indicate no significant difference. C The relative (%) inhibitory effect of 2-DG on LCMV infection was calculated from LCMV titers shown in B (for more details, see Materials and methods). Data from four independent experiments are shown as a mean and SD. FFU/ml, focus-forming units per milliliter