Fig. 3. Immunophenotyping of CD8⁺ T lymphocytes in immunized mice.

After 90 days of immunization, mice splenocytes were stimulated with TRASP for 18 h or left unstimulated (RPMI only) and were then characterized by flow cytometry. a UMAP projection of IFN-γ production by CD8⁺ T lymphocytes compartments, separated in Naïve (CD8⁺ CD44⁻ CD62L⁻), effector/effector memory (CD8⁺ CD44⁺ CD62L⁻), and central memory (CD8⁺ CD44⁺ CD62L⁺). b, c The frequency of IFN-γ-producing CD8+ effector/effector memory (b) or central memory T cells (c). The statistical analysis was carried out using paired t-test. Data were representative of two independent experiments (n = 3 mice per group). ns non-significant.