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. Author manuscript; available in PMC: 2023 Aug 8.
Published in final edited form as: Biomacromolecules. 2022 Jul 5;23(8):3116–3129. doi: 10.1021/acs.biomac.2c00048

Figure 3.

Figure 3.

Expression of 5FA and X-5FA was verified along with phase behavior. (A) 5FA and X-5FA constructs were purified by thermal cycling. The approximate size and purity of ELP constructs were verified by SDS–PAGE. (B) NHS-rhodamine consistently labeled these ELPs, enabling fluorescence imaging. (C) The transition characteristics of 5FA and X-5FA were compared at 5 μM showing a slight trend of lower Tt of X-5FA constructs relative to untargeted 5FA. (D) Higher concentration of all ELP constructs indicated an inverse relationship with Tt in a range between 0.3 and 5 μM. Based on the fit to the OD data, Tt was calculated to be above physiological temperatures at all concentrations in the tested range up to 5 μM.