Figure 4. HNF4α2 is a direct transcriptional regulator of osteogenesis and metabolism in osteoblasts.

(A) Canonical pathway analysis of HNF4α targets identified by ChIP sequencing of Ctr, Hnf4α1^Tg, and Hnf4α2^Tg osteoblasts. n = 3 biological replicates per experimentally used antibody. (B) Number of genes differentially regulated in Hnf4α1^Tg and Hnf4α2^Tg osteoblasts versus Ctr and directly regulated by HNF4α, obtained from the intersection between genes identified by RNA-Seq in Figure 2 and genes identified by ChIP sequencing in Figure 3. (C) Canonical pathway analysis and prediction of pathway activation of direct HNF4α targets identified in A.