Figure 6. Low bone mass is associated with altered osteogenesis and impaired bone metabolism in Hnf4α^Oc-cKO mice.

(A) Canonical pathway analysis of differentially regulated genes identified by RNA-Seq of bone from 6-week-old Hnf4α^Oc-cKO mice versus WT. (B–D) Heatmap-represented, log-normalized expression of genes identified in the topmost differentially regulated pathways in Hnf4α^Oc-cKO bone versus WT. (E) Number of genes differentially regulated in bone in Hnf4α^Oc-cKO versus WT identified by RNA-Seq and directly regulated by HNF4α1 or HNF4α2, obtained from the intersection with previously identified direct HNF4α targets in osteoblast ChIP sequencing in Figure 4B. (F) Canonical pathway analysis of direct HNF4α1 and HNF4α2 gene targets in bone identified in E. In A and F, prediction of pathway activation is indicated by z score on the heatmap. n = 4 per group; corrected P < 0.05 versus WT. Statistical analysis was performed with unpaired Student’s t tests and corrected by the FDR.