Figure 7. Osteoblast-specific deletion of Hnf4α alters osteoblast differentiation and function.

(A–C) Alkaline phosphatase (ALP) (A) and alizarin red S (ARS) (B) staining and quantification and mRNA expression of Hnf4α and osteoblastic markers (C) in differentiated primary BMSC cultures isolated from 6-week-old WT and Hnf4α^Oc-cKO mice. (D–F) ALP (D) and ARS (E) staining and quantification and mRNA expression of Hnf4α and osteoblastic markers (F) in differentiated primary mature osteoblast cultures isolated from 6-week-old WT and Hnf4α^Oc-cKO mice. Values are expressed as the mean ± SEM. n ≥ 3 per group of a representative experiment performed at least 3 times; P < 0.05 versus *WT. Statistical analysis was performed with an unpaired Student’s t test.