Figure 8. Bone Hnf4α expression is reduced in mice with CKD and in response to acute and chronic inflammation.

(A) Renal function in 20-week-old sham-operated and bilateral ischemia/reperfusion injury (bIRI) WT male mice assessed by measurements of blood urea nitrogen (BUN). (B) Bone Hnf4α expression levels in 20-week-old sham and bIRI male mice. (C–H) Microtomography analysis of femur metaphysis secondary spongiosa (C–E) and femur cortical bone at metaphysis (F–H) in 20-week-old WT sham and bIRI mice. Ct, cortical; Po, porosity. Values are expressed as the mean ± SEM. n ≥ 4 per group; P < 0.05 versus *sham. (I and J) Hnf4α mRNA expression in a representative experiment performed at least 3 times in differentiated primary BMSC cultures isolated from WT mice treated for 6 hours with different concentrations of IL-1β, PTH, inorganic phosphate salts, IL-6, or TNF-α. (K and L) mRNA expression of bone Hnf4α in tibiae from WT mice injected with saline (Ctr), IL-1β, or heat-killed Brucella abortus (BA) 6 hours (K) or 14 days (L) after injection. Values are expressed as the mean ± SEM. n ≥ 4 per group; corrected P < 0.05 versus *Ctr. Statistical analysis was performed with an unpaired Student’s t test (A–H and L) or with an ANOVA followed by post hoc t tests and multiple-testing correction using the Holm-Bonferroni method (I–K).