Fig. 8. Inhibition of abnormal neovascularization by diacetylchrysin. The db/db mice were orally supplemented with 10 mg/kg diacetylchrysin daily for 10 weeks. The db/m mice were employed as control animals. To reveal acellular capillaries (red arrow) in db/db mice treated with 10 mg/kg diacetylchrysin, retinal vessels were subjected to H&E staining (A). Magnification: 200-fold. To detect new capillary bed formation (yellow arrow) and vessel leakage (red arrow) by diacetylchrysin exposure (B), typical appearance of retinal capillaries was observed by FITC-dextran-perfused retinal flat-mounts. Retinas were dissected, flat mounted, and observed by confocal microscopy. Magnification: 400-fold. Each photograph is representative of 4 mice.

H&E, hematoxylin and eosin; FITC, fluorescein isothiocyanate.