Figure 3.

RT-QuIC CSF end point dilutions for E200K-AR and E200K converter samples to calculate SD₅₀/ µl. Each panel series show dilutions of seeding E200K CSF volume by a third; the vertical dotted line indicates the time cut-off, which was 24 h for IQ-CSF RT-QuIC. (A and B) From a single individual drawn at 3.75 and 1.70 years from the present, respectively. (C and D) From a single converter individual 0.6 years apart at 0.2 years to, and 0.4 years after conversion, respectively. The dotted vertical lines indicate the time cut-offs for the individual assays i.e. 24 h.