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. 2023 Apr 20;24(6):915–924. doi: 10.1038/s41590-023-01493-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — (a) Representative visualization of Mcpt6 mRNA using fluorescence in situ hybridization (FISH) in ear skin whole mount tissue of adult WT mice. These data refer to the post-imaging analysis of Mcpt6 expression in periarteriolar versus interstitial MCs in Fig. 4i,j. Representative image from one of n = 4 mice. (b) Differential expression of MCPT6 in periarteriolar versus interstitial MCs. Ear skin whole mount tissue of an adult WT mouse is counter-stained for the pan-MC marker avidin (cyan), MCPT6 (green), COL4-expressing basement membranes (red) and ACTA2-positive arterioles (white). Avidin and MCPT6 fluorescence intensities are also displayed as heatmap. (c,d) Imaris software-based workflows for the post-imaging analysis of MCPT6 mean fluorescence intensities in MC subsets (c) and calculation of the percentage of MCPT6^high MCs of all periarteriolar MCs in WT and Tln1^ΔMC mice (d). (b-d) Representative images from one experiment of n = 5 mice. Scale bars: 50 µm (a, d), and 100 µm (b,c).