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. 2023 May 31;14:3150. doi: 10.1038/s41467-023-38841-7

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A–C Tumor growth of CTRL and Adam2 O/E LLC cells allografted subcutaneously into C57BL/6 mice (A, n = 4 CTRL, n = 5 Adam2), NSG mice (B, n = 8) and Nude mice (C, n = 5) examined over three independent experiments. The p values for tumor growth were determined by an unpaired two-sided t-test with Welch’s correction. The data are presented as the mean ± SEM. D Volcano plot showing differentially expressed genes (DEGs) in Adam2 O/E compared to CTRL tumors isolated from C57BL/6 mice, where log2 FC indicates the mean expression and (−)log10 adjusted p-value level for each gene. The blue dots denote downregulated gene expression, the red dots denote upregulated expression, and black dots denote the gene expression without marked difference. Data represents three independent biological samples for each group. E GSEA plots showing Hallmarks of IFNγ and IFNα pathways between Adam2 O/E compared to CTRL tumors isolated from C57BL/6 mice (A) with FDR < 25% and p < 1%. F Bar graph showing Gene Ontology of the DEGs (FC > 2, p < 0.05) downregulated in Adam2 O/E compared to CTRL tumors assigned to Biological Process isolated from C57BL/6 mice (A). G Bar graph depicting selected downregulated genes in Adam2 O/E compared to CTRL tumors categorized by immune function isolated from C57BL/6 mice (A). H Cell surface expression of MHC-I H2K^b-OVA on CTRL and Adam2 O/E LLC cells after treatment with 100 ng/ml of IFNγ, IFNβ or TNFα over the indicated time course. Numbers denote mean fluorescence intensity (MFI). I Percentage of CTRL and Adam2 O/E LLC cells expressing CD74 and Tigit after IFNγ, IFNβ or TNFα treatment (100 ng/ml each, 24 h). Data presents mean ± s.e.m. of three technical replicates analyzed by two-sided student’s t-test examined. One representative experiment out of three independent experiments is shown.