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SEC-MALS profiles of vimentin tetramers and its progressive truncations on a Superdex 200 Increase column. Across each elution peaks, MALS-derived molecular weight profiles are also shown (right axis). (A) Elution of FL vimentin in LS buffer (2 mM HEPES, pH 8.2). (B) Elution of different truncations in HS buffer (10 mM HEPES, 150 mM NaCl, pH 7.5).
