Table 1.
Oligomerization properties of vimentin constructs in HS conditions.
Name | Residues | Method | Theoretical monomer mass (kDa) | Experimental mass (kDa) | Peak elution position (mL) | Oligomeric state |
---|---|---|---|---|---|---|
Rod | 93–404 | SEC-MALS | 36.9 |
149 n.d |
9.6 8.6 |
Tetramer Oligomers |
XL + gel | Tetramer | |||||
ΔNrod | 150–404 | SEC-MALS | 30.2 |
57 126 |
11.6 9.9 |
Dimer Tetramer |
XL + gel | Dimer | |||||
Coil1B | 150–249 | SEC-MALS | 12.1 | 25 | 14.4 | Dimer |
XL + gel | Dimer |