Table 2.
Segments (# XLs) | Residue 1 | Residue 2 | Cross-linker(s) | Type XL | Elongated model, Cα-Cα dist. (Å) | Compact model, Cα-Cα dist. (Å) |
---|---|---|---|---|---|---|
C1B–C1B (2) | K143 | K223 | DSG, DSS,*,** | inter | 29 | 32 |
K188 | K188 | DSG, DSS, DSPU, DSBU,* | inter | 16 | 16 | |
C1A–C1B (1) | K120 | K168 | DSS | intra | 69 | 34 |
C1B–C2 (4) | K143 | K294 | DSS | inter | 35 | 30 |
K188 | K334 | DSBU | intra | 169 | 23 | |
K223 | K294 | DSG, DSS, DSBU | intra | 61 | 28 | |
K223 | K313 | DSG | intra | 88 | 31 | |
C2–C2 (1) | K294 | K402 | DSBU,*,** | inter | 369 | 62 |
C1A–C2 (15) | K97 | K120 | DSS, DSBU | intra | 33 | 33 |
K97 | K292 | DSG | inter | 39 | 25 | |
K97 | K294 | DSG, DSS, DSBU | inter | 36 | 25 | |
K97 | K313 | DSS | inter | 32 | 24 | |
K97 | K402 | DSBU | intra | 404 | 85 | |
K104 | K282 | DSS,*,** | inter | 43 | 33 | |
K104 | K292 | DSS | inter | 30 | 21 | |
K104 | K294 | DSS | inter | 27 | 19 | |
K104 | K313 | DSG, DSS | inter | 16 | 19 | |
K120 | K262 | DSG,*,** | inter | 28 | 39 | |
K120 | K282 | DSG, DSS, DSBU | inter | 22 | 17 | |
K120 | K292 | DSS, DSBU | inter | 13 | 14 | |
K120 | K294 | DSS, DSBU | inter | 15 | 15 | |
K120 | K313 | DSS, DSBU | inter | 31 | 36 | |
K129 | K294 | DSG, DSS | inter | 16 | 28 | |
(23 XLs) | (17 satisfied) | (21 satisfied) |
Asterisks indicate same or nearby cross-linked positions as reported by Steinert’s group for DST in23 (*) and9 (**). The last two columns indicate the distances between the corresponding residues in each of the models. The distances which are compatible with XL formation are in bold. The distances which exceed the cut-off value are in italics.