Table 4.

‘In vitro’ factors and variables potentially influencing SDT outcomes

Factors	Variables
Ultrasound Parameters	• Frequency
	• Intensity
	• Irradiation time and duty cycle
Spatial Configuration	• Cell-to-transducer distance
	• Ultrasound beam/culture vessel ratio
Coupling Media	• Volume
	• Composition
	• Viscosity
	• T°
	• Acoustic Propagation
Culture Vessels	• Geometry
	• Absorbers
	• Material Type
Culture Medium	• Composition
	• Volume
	• Acoustic Properties
Irradiation sample type	• Adhering cells
	• Suspension cells
	• Cell motion