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. 2023 Mar 31;28(5-6):881–891. doi: 10.1007/s10495-023-01828-2

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Fig. 2 — Weakened autophagic level of CAFs induced by 3-MA suppressed proliferation, migration and invasion of PCa cells

CAFs were pretreated with autophagy inhibitor 3-MA. (A) Western bolt detection of autophagy- related proteins. (B-E) CM was collected from NFs or CAFs with or without 3-MA pretreatment, then PCa cells were incubated with NFs-CM or CAFs-CM. (B) The schematic illustration of CM collection and PCa cells incubation with CM. (C) Cell viability was evaluated using CCK-8 assay. (D) Representative pictures and the quantification of wound closure rates in wound healing assay. (E) Transwell assay for cell invasion measurement. Data are presented as mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001