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. 2023 May 18;14:1181876. doi: 10.3389/fimmu.2023.1181876

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Copyright © 2023 Yount, Hall, Caution, Shamseldin, Guo, Marion, Fullen, Huang, Maynard, Quataert, Deora and Dubey

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prime-pull immunization with aPV elicits Th2 polarized CD4+ T_RM C57BL/6 mice (5/group) were immunized on d0 and d28 with 1/5 human dose aPV delivered i.m./i.m. or i.m./i.n. T_RM in lungs and nose were quantified via flow cytometry on a Cytek Aurora spectral flow cytometer as live, CD3+, CD4+, CD45 i.v.-, CD44+, CD62L-, CD69+ cells. Number of CD69+ T_RM in (A) lungs and (B) nose. Number of CD69+ T_RM in the lungs and nose that were IFNγ+ (C), IL-17+ (D), or IL-5+ (E) following PMA/I stimulation. Data representative of two independent experiments and presented as mean +/- SEM. Data were analyzed by one-way ANOVA with Holm-Sidak correction for multiple comparisons. *P<0.05.