Figure 5.
Prime-pull immunization with aPV/BcfA reduces B. pertussis burden in the nose upon challenge. C57BL/6 mice were primed on d0 and boosted on d28 with the indicated vaccines and immunization routes. Unimmunized and immunized mice were challenged i.n. on d42 with 5x105 CFU Bp536. (A) Lungs and (B) nose were harvested at the indicated time points and CFUs enumerated on BG agar plates. 5 mice/group. Dotted line represents limit of detection (LOD) for lung at 20 CFU. LOD for nose is 30 CFU (below the scale). Data were analyzed by two-way ANOVA with Holm-Sidak correction for multiple comparisons. Black asterisks indicate significant differences between unimmunized mice and the immunization groups at all time points post-challenge. The brackets indicate statistical differences between i.m./i.m. aPV and i.m./i.n. aPV/BcfA at day 4 and day 7 post-challenge. ***P<0.001, ****P<0.0001 by ANOVA.