Figure 2.

Hyal-2 increases L929 cell sensitivity to TNF cytotoxicity. L929 cell lines that stably expressed GFP and GFP-Hyal-2 were established (see Materials and Methods) and exposed to TNF-α, staurosporine, actinomycin D (ActD), daunorubicin, or camptothecin for 24 hr. The extent of cell death were measured by staining the cells with crystal violet as previously described (mean ± standard deviation; n = 8) [10].