Figure 2.

High-throughput screening identifies readthrough modulators. (A) Schematic illustration of Aqp4 readthrough. (B) Screening procedure. Transfected cells were seeded into wells pre-spotted with compounds, DMSO (no-treatment control) or Geneticin (positive control). The last column received control cells in which the Aqp4 stop in the vector was mutated to a sense codon. (C) Separation bands between the no-treatment and positive controls and a summary of Z-factors for the plates used in screening. Dots and error bars represent means and 3 SD, respectively. (D) Quantile analysis of the screening results. (E) Compounds enhancing (solid bars) or inhibiting (patterned bars) FL/RL values by at least 50%. The change in FL/RL is largely due to the modulation of FL, not RL.