Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 19;163(11):bqac154. doi: 10.1210/endocr/bqac154

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

PMC Copyright notice

Figure 4. — LAMA4 negatively regulates thermogenic gene expression in human adipocytes. A, Representative images outlining differentiation and treatment trajectory of human induced pluripotent stem cells (hiPSCs) to beige adipocytes. Images were taken at 20× magnification. B, Relative messenger RNA (mRNA) expression of thermogenic genes UCP1, PRDM16, and CIDEA (n = 7), and adipogenic genes PPARG and ADIPOQ (n = 4) in hiPSCs on day 4 and day 16 of beige adipocyte differentiation. Reported as fold change with respect to paired control. *, **, ***, and **** indicate P less than or equal to .05, .01, and .001, 0.0001, respectively. Data are means + SD. C, Relative mRNA expression of thermogenic genes UCP1, PRDM16, and CIDEA (n = 7) in human beige adipocytes treated with or without LN411 during differentiation. D, Protein expression of UCP1 in human beige adipocytes treated with or without LN411 during differentiation (n = 5). Beta actin was used as the loading control for normalization. E, Protein expression of mitochondrial respiratory chain complexes I, II, III, and V in human beige adipocytes treated with or without LN411 during differentiation (n = 5). Vinculin was used as the loading control for normalization. F, Protein expression of AMPKα in human beige adipocytes treated with or without LN411 during differentiation (n = 5). GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was used as the loading control for normalization.