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. 2022 Sep 19;163(11):bqac154. doi: 10.1210/endocr/bqac154

Figure 5.

Figure 5.

Silencing of ILK in murine adipocytes elevates thermogenic gene expression. A, Relative Ilk messenger RNA (mRNA) expression (WT n = 9, Lama4−/− n = 8) and ILK protein expression (WT n = 4, Lama4−/− n = 4) in sWAT of male mice. Reported as fold change with respect to average of WT mice after normalization. *, **, ***, and **** indicate P less than or equal to .05, .01, .001, and .0001, respectively. Data are means + SD. B, Relative Ilk mRNA expression (n = 6) and ILK protein expression (n = 4) in adipocytes treated with control small interfering RNA (siRNA) and Lama4 siRNA. Reported as fold change with respect to paired control. C, Relative Ilk mRNA expression (n = 6) and ILK protein expression (n = 5) in adipocytes treated with control siRNA and Ilk siRNA. Reported as fold change with respect to paired control. D, Relative Ucp1, Tbx1, Prdm16, and Cidea mRNA expression in adipocytes treated with control siRNA (n = 6) and Ilk siRNA (n = 6). E, Protein expression of UCP1 in adipocytes treated with control siRNA (n = 5) and Ilk siRNA (n = 5) was assessed by Western blot. Vinculin was used as the loading control for normalization. F, Relative Cpt1a and Cpt1b mRNA expression in adipocytes treated with control siRNA (n = 6) and Ilk siRNA (n = 6). G, Protein expression of mitochondrial respiratory chain complexes I, II, III, and V, and VDAC in adipocytes treated with control siRNA (n = 5) and Ilk siRNA (n = 5) was assessed by Western blot. Vinculin was used as the loading control for normalization.