Fig. 2.

Screening of the diamide masarimycin against Streptococcus pneumoniae . (a) The diamide masarimycin inhibits detergent-induced autolysis in a concentration dependent manner. Percent residual activity was calculated using autolysis in the absence of inhibitor set as 100 %. Data shown is the average of experiments performed in biological and technical triplicate. Error bars denote standard deviation. (b) Activity of masarimycin against S. pneumoniae R6 autolysin and cell wall biosynthesis mutants (13) to identify changes to masarimycin sensitivity. Assays were run in biological triplicate and yielded the same MIC values.