Table 1.

Overexpressed proteins observed in SDS-PAGE gel (Fig. S5b) of S. pneumoniae R6 cell-surface protein extracts when exposed to 0.75 x MIC masarimycin

Gel band	Protein ID	Biological function
1	BgaA β-galactosidase Iga immunoglobin A1 protease	Plays role in growth and adhesion [65] Covalently linked to cell surface by sortase A. Involved in host immune evasion [66].
2	Spr0440, endo-β-GlcNAcase	Surface protein, role in commencement of neuroinvasion [67]
3	Elongation factor Tu PykF Pyruvate kinase	Moonlighting function in regulation of cell shape [68] Moonlighting protein [69, 70]
5	MalX maltooligosaccharide transporter	Complex carbohydrate catabolism regulated by CiaR/H system which is involved in sensing cell wall stress [46, 71].
6	GapA glyceraldehyde 3-phosphate dehydrogenase	Moonlighting protein [70]