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. 2022 Jun 29;145(9):3250–3263. doi: 10.1093/brain/awac152

Figure 5.

Figure 5

Adnp or Pogz deficiency in mouse PFC increases microglia and astrocytes without changing neuron numbers. (A–D) Top: representative images of microglial marker Iba1 (red, A), activated microglial marker Cd68 (red, B), astrocyte marker Gfap (red, C) or neuronal marker NeuN (red, D) in PFC slices from mice with the injection of a control shRNA, Adnp shRNA or Pogz shRNA AAV. Green, AAV-linked GFP signal. Bottom: bar graphs of Iba1 (A), Cd68 (B), Gfap (C) or NeuN (D) integrated density (mean fluorescence intensity × area of fluorescence) and average number of Iba1+ (A), Cd68+ (B), Gfap+ (C) or NeuN+ (D) cells per image area (0.085 mm2) in each group (Iba1, n = 3 animals/18 images (control); 4 animals/19 images (Adnp); 4 animals/18 images (Pogz); density: F(2,52) = 6.71, P = 0.0026, number: F(2,52) = 11.96, P < 0.0001; Cd68, n = 2 animals/7 images (control); 3 animals/9 images (Adnp); 3 animals/10 images (Pogz); density: F(2,23) = 5.62, P = 0.010, number: F(2,24) = 8.84, P = 0.0013; Gfap, n = 3 animals/15 images (control); 4 animals/16 images (Adnp); 4 animals/19 images (Pogz); density: F(2,47) = 11.57, P < 0.0001, number: F(2,47) = 16.41, P < 0.0001; NeuN, n = 3 animals/12 images (control); 4 animals/15 images (Adnp); 4 animals/16 images (Pogz); density: F(2,40) = 1.18, P = 0.32, number: F(2,40) = 1.67, P = 0.20, one-way ANOVA). All data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.