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. 2022 Jun 29;145(9):3250–3263. doi: 10.1093/brain/awac152

Figure 6.

Figure 6

Adnp or Pogz deficiency in mouse PFC leads to diminished glutamatergic transmission and postsynaptic protein expression. (A and B) Bar graphs of spontaneous excitatory postsynaptic current (sEPSC) frequency and amplitude in PFC pyramidal neurons from mice with injection of a control shRNA, Adnp shRNA (A) or Pogz shRNA (B) AAV [A: n = 3 animals/group, cells: n = 9 (control), n = 10 (Adnp); t = 2.47, P = 0.027 (frequency); t = 0.408, P = 0.689 (amplitude); B: n = 4 animals/group, cells: n = 20 (control), n = 19 (Pogz); t = 3.28, P = 0.0021 (frequency); t = 1.65, P = 0.108 (amplitude), unpaired t-test]. Inset: representative sEPSC recordings from each group. (C) Representative AMPAR-EPSC (recorded at −70 mV) and NMDAR-EPSC (recorded at +40 mV) evoked on the same PFC pyramidal neurons from each group. (D and E) Bar graphs of AMPAR-EPSC amplitudes (D) and NMDAR-EPSC amplitudes (E, detected at 40 ms after current onset) in each group (n = 3–4 animals/10–29 cells/group, AMPA: F(2,55) = 11.43, P < 0.0001, NMDA: F(2,55) = 5.01, P = 0.01, one-way ANOVA). (F–I) Bar graphs comparing expression levels of synaptic proteins PSD95, GluR1, NR1 and Snap25 in PFC synaptic fraction from mice with injection of a control shRNA, Adnp shRNA or Pogz shRNA [n = 5–7 animals/protein, control; n = 6 (Adnp); n = 6 (Pogz); one-way ANOVA]. All proteins are normalized to actin. (J) Representative western blot images from each group. All data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.