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. 2023 Mar 28;7(3):100133. doi: 10.1016/j.rpth.2023.100133

Figure 1.

Figure 1

Analysis of Innovin by western blotting. Recombinant (r) tissue factor (TF) (Innovin) was analyzed by western blot analysis (1-1120 pg/lane). Cell lysates from a high TF leukemia cell line (NB4, 40 μg/lane) and a low TF leukemia cell line (HL-60, 40 μg/lane) and were used as controls. An antihuman TF polyclonal antibody from R&D Systems (#AF2339) was used (1:1000 dilution in blocking buffer, final concentration 0.2 μg/mL). The secondary antibody was used at a 1:5000 dilution in blocking buffer. Molecular weight (MW) standards are shown. The position of rTF (non-glycosylated, ∼40kDa) and TF (glycosylated, ∼50kDa) are shown. The arrowhead indicates the position of a TF dimer.