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. 2023 Mar 28;7(3):100133. doi: 10.1016/j.rpth.2023.100133

Figure 3.

Figure 3

Measurement of tissue factor in extracellular vesicles isolated from cancer cell culture supernatant. Extracellular vesicles (EVs) were isolated from a tissue factor (TF)-positive pancreatic cancer cell line (HPAF-II) by centrifuging 100 μL of cell culture supernatant at 20,000 g for 15 minutes at 4 °C. The pellet was resuspended in 100 μL of PBS. (A) TF activity was determined using a FXa generation assay. EV TF antigen was determined using (B) Imubind ELISA, and (C) Quantikine ELISA.