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. 2023 Mar 28;7(3):100133. doi: 10.1016/j.rpth.2023.100133

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Measurement of tissue factor activity and antigen in human plasma from whole blood with or without lipopolysaccharide stimulation. Whole blood from 5 healthy donors was left untreated or stimulated with lipopolysaccharide (LPS) and plasma prepared. (A) Extracellular vesicles (EVs) were isolated from plasma by centrifuging 100 μL of plasma and resuspended in 100 μL of assay buffer. Tissue factor (TF) activity was determined using a FXa generation assay. TF antigen in plasma from unstimulated or LPS-stimulated whole blood was determined using (B) Imubind ELISA, (C) Quantikine ELISA, (D) SimpleStep ELISA, and (E) CD142 ELISA.