Figure 5.

Measurement of tissue factor antigen in plasma, extracellular vesicle-free plasma, and extracellular vesicles from whole blood with or without lipopolysaccharide stimulation. Whole blood from 4 healthy donors was left untreated or stimulated with lipopolysaccharide (LPS) and plasma was prepared. Extracellular vesicles (EVs) were isolated from plasma by centrifuging 100 μL of plasma at 20,000 g for 15 minutes at 4 °C. The pellet was resuspended in 100 μL of sample buffer. EV-free plasma was generated by centrifuging the supernatant at 100,000 g for 70 minutes at 4 °C. Tissue factor (TF) antigen in plasma, EV-free plasma (EV-free) and EVs was determined using (A) Imubind ELISA, and (B) Quantikine ELISA.