Fig. 5.

NRN1 protects against Aβ₄₂-induced neuronal hyperexcitability.A, schematic representation of primary rat hippocampal neuron treatment and single neuron electrophysiology analysis. B, representative raster plots from three units after exposure to DMSO, 500 nM Aβ₄₂, 150 ng/ml NRN1, or 150 ng/ml NRN1 and 500 nM Aβ₄₂. C, mean firing rate at DIV14 in hippocampal neurons treated with DMSO, compared to baseline (n = 36–54 neurons, unpaired Student’s t test; p = 0.1296). D, mean firing rate at DIV14 in hippocampal neurons treated with 500 nM Aβ₄₂, compared to baseline (n = 65–68 neurons, unpaired Student’s t test; p = 0.0022). E, mean firing rate at DIV14 in hippocampal neurons treated with 150 ng/ml NRN1, compared to baseline (n = 32–33 neurons, unpaired Student’s t test; p = 0.0023). F, mean firing rate at DIV14 in hippocampal neurons treated with 150 ng/ml NRN1 and 500 nM Aβ₄₂, compared to baseline (n = 100–107 neurons, unpaired Student’s t test; p = 0.0676). Aβ, amyloid-beta; NRN1, neuritin.