Fig. 6.

NRN1 treatment induces changes in the neuronal proteome related to broad synaptic functions.A, schematic representation of rat primary cortical neuronal culture workflow in which neurons were maintained in neurobasal medium for 14 days, treated with 150 ng/ml of NRN1 and analyzed via TMT-MS. B, differential protein expression between NRN1-treated and vehicle-treated neurons (n = 8238 proteins). Proteins above the dashed line (p = 0.05) are considered significantly differentially expressed. Student’s t test was used to calculate p values. Large spots are based on reproducibility-optimized test statistic (ROTS) correction of differentially expressed proteins with 10% or less false discovery rate (FDR). C, gene ontology of significantly differentially expressed proteins in NRN1 treated neurons. A Z-score above 1.96 was considered significant (p < 0.05). NRN1, neuritin; TMT-MS, tandem mass tag mass spectrometry.