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RT-qPCR results after 3 min synthetic CSP treatment. Treatment with exogenous CSP for 3 min leads to activation of the comX gene. After a 3 min incubation, stop solution was added to each sample to stop transcription. Data are normalized to expression of gyrB. The RT-qPCR experiment was conducted using two biological replicates for both the CSP and DMSO conditions.
