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S. sinensis transformation assay. S. sinensis demonstrates dependence on the addition of exogenous synthetic CSP for the successful uptake of added plasmid (pDL278). The synthetic CSP was added at final concentrations of 10 000 nM (a), 1000 nM (b), 100 nM (c) and 10 nM (d). The control includes no exogenous CSP (e). See the Methods section for full experimental details.
