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. 2023 Mar 3;21(6):614–627. doi: 10.1158/1541-7786.MCR-22-0370

Figure 1.

Figure 1. JMJD6 regulates ERα activity and affects prognosis in luminal patients with breast cancer. A, Kaplan–Meier analysis of patients with breast cancer included in the METABRIC dataset (available on cBioportal) showing the OS of all (left, n = 1,884), ERα-positive (middle, n = 1,440) or ERα-negative patients (right, N = 444). ‘High’ (red) and ‘low’ (blue) JMJD6 expression is shown according to median. P value was calculated with Log-rank (Mantel-Cox) test. B, Western blot showing the expression of JMJD6 and ERα in control (SCR) and JMJD6 KO MCF7 and MDA-MB231 clones. Vinculin is shown as loading control. C, Immunofluorescence staining of ERα (red) in scramble or JMJD6 KO MCF7 cells stimulated or not with E2. Nuclei were stained with DAPI (blue), bars = 50 μmol/L. D, Real Time PCR to evaluate the levels of ESR1 and (E) its target genes (PGR, BCL2, XBP1 and RARA) in control (SCR) of JMJD6 KO MCF7 stimulated or not with E2. Statistical significance was calculated by paired two-tailed t test comparing four independent experiments.

JMJD6 regulates ERα activity and affects prognosis in luminal patients with breast cancer. A, Kaplan–Meier analysis of patients with breast cancer included in the METABRIC dataset (available on cBioportal) showing the OS of all (left, n = 1,884), ERα-positive (middle, n = 1,440) or ERα-negative patients (right, N = 444). ‘High’ (red) and ‘low’ (blue) JMJD6 expression is shown according to median. P value was calculated with Log-rank (Mantel-Cox) test. B, Western blot showing the expression of JMJD6 and ERα in control (SCR) and JMJD6 KO MCF7 and MDA-MB231 clones. Vinculin is shown as loading control. C, Immunofluorescence staining of ERα (red) in scramble or JMJD6 KO MCF7 cells stimulated or not with E2. Nuclei were stained with DAPI (blue), bars = 50 μmol/L. D, Real Time PCR to evaluate the levels of ESR1 and (E) its target genes (PGR, BCL2, XBP1 and RARA) in control (SCR) of JMJD6 KO MCF7 stimulated or not with E2. Statistical significance was calculated by paired two-tailed t test comparing four independent experiments.