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. 2023 Mar 14;42(11):e111901. doi: 10.15252/embj.2022111901

Figure EV1. Genetic perturbation of mitochondrial morphology has selective effects on mitochondrial substrate oxidation.

Figure EV1

  • A
    Quantification of phosphorylating ADP and maximal respiration induced by palmitoyl‐CoA in permeabilized HepG2 cells. n = 5 independent biological replicates for siCtrl and n = 3 independent biological replicates with six technical repeats for all other perturbations. Data represent means ± SEM, t‐test. *P < 0.05.
  • B
    Succinate‐induced respiration in permeabilized HepG2 cells. n = 3 biological replicates with six technical repeats. Data represent means ± SEM, t‐test. *P < 0.05.
  • C
    Representative images of MFN1 KO HepG2 cells and quantification of mitochondrial length. Scale bar: 10 μm. Data represent means ± SEM, n = 3, t‐test. *P < 0.05.
  • D–F
    Quantification of CPT1 protein expression in HepG2 cells following expression of DN‐DRP1 (D), excess nutrient treatment (E), or miMFN2 (F).