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. 2023 Apr 21;42(11):e113578. doi: 10.15252/embj.2023113578

Figure EV4. Structural characterization of the VP40 matrix in VLPs by cryo‐ET.

Figure EV4

  1. VP40 dimer structure (pdb: 7jzj) fitted into the subtomogram average presented in Fig 2 from the side view including the density of the inner VLP monolayer and rotated by 90°. Helical segments protruding from the subtomogram average are highlighted in shades of orange.
  2. Diameter of VLPs composed of GP and VP40 measured from membrane‐to‐membrane after incubation at pH 7.4 and pH 4.5. Asterisks indicate statistical significance as judged by a two‐tailed Welch's t‐test, assuming unequal variance (P < 0.0001).
  3. Quantification of the VP40 assembly state of VLPs composed of VP40 and GP (n = 37 at pH 7.4 and 18 at pH 4.5); or VP40 alone (n = 22 at pH 7.4 and 8 at pH 4.5). The VP40 matrix was either assembled (blue), or disassembled (dark pink). VP40 assembly in VLPs subjected to neutral or low pH was assessed by cryo‐ET.

Data information: Scale bars: (A) 2.5 nm.