Fig. 2.

Gellification of droplets retain the efficiency of vddPCR. a qPCR readout of vddPCR and gc-dPCR suggested that both vddPCR and gc-dPCR are more sensitive than qPCR. b Endpoint measurement of each assay. vddPCR and gc-dPCR produced stronger fluorescence intensity compared to qPCR. The boxplots represent statistical analysis of data obtained from 6 independent tests. Statistical significance was evaluated by one-way analysis of variance followed by post-hoc Tukey honest significance difference test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns indicates not significant