Fig. 1.

Working principle of tissue slice culture. A The sample is embedded in agarose and sticked to the block holder (left). B Side-view of a typical vibratome cutting chamber (right). C Typical culture methods employed downstream of slice generation: submerged in culture medium, on insert submerged or at the air–liquid interface, in each case with or without perfusion, and in a microfluidic culture chamber. Figures were created using Krita (https://krita.org)