Table 3.

Non-exhaustive selection of studies culturing vibrating microtome-generated slices from different tumor types

Tumor type	Species	Slice thickness	Culture time (max)	Culture system features	Purpose	Ref
Lung	hu	300 µm	6 months	Implanted into mice (xenograft)	Model for primary tumor expansion and xenograft production	[69]
	sh, ms	300 µm	1 month	Submerged	Standardized slice model for viral infection gene therapy	[70]
	ms	160-250 µm	3 days	ALI, titanium grid, rotation	Tumor drug testing model	[71]
Oral squamous cell carcinoma	hu	350–450 µm	8 days	Chorioallantoic membrane (CAM)	Establishing slices on CAM as a tumor model	[72]
Gastrointestinal (various)	hu	250 µm	7 days	On insert, submerged	Protocol, method evaluation	[73]
Prostate	hu	200-300 µm	10 days	Submerged, hypoxia	Prostate tumor model	[59]
	ms	300 µm	6 days	With/without insert and strainer	Establishing a chemotherapy model	[74]
	hu	250 µm	9 days	On insert, ALI	Model for immune microenvironment studies	[75]
	hu	350 µm	96 h	On insert, submerged	Model development	[76]
	hu	350 µm	96 h	On insert, submerged	Assessing culture effects by transcriptome profiling	[77]
	hu	250 µm	9 days	On insert, ALI	Model development	[78]
	hu	250 µm	4 days	On insert, ALI	Tumor immunology studies	[79]
	hu	300 µm	15 days	On insert, ALI	Method development	[80]
	hu	250 µm	6 days	On insert, submerged	Interaction of tumor cells with immune microenvironment	[81]
Liver	hu	200-300 µm	3 days	Submerged	Comparison of slicing devices	[82]
	hu, ms	200-300 µm	7 days	On insert, submerged	Drug discovery, immuno-oncology	[83]
	hu	250 µm	4 days	On insert, submerged	Immune checkpoint ligands and chemotherapy response	[84]
	hu	250 µm	6 days	On insert, submerged	Establish CarT-cell treatment model	[85]
	hu	250 µm	3 days	On insert, submerged	Neutralizing antibodies and CAR-T cells in cancer therapy	[86]
Bladder	hu	300 µm	2 days	On insert, submerged, on a rotating plate	Method for studying oncolytic viruses	[87]
Kidney	hu	300 µm	1 week	Submerged	Characterization of the tumor immune environment	[88]
Uterine leiomyoma	hu	500 µm	3 weeks	On alginate scaffold discs	Model development	[89]
Breast	hu	300 µm	7 days	Submerged with/without rotating platform	Model development, comparison with manual slicing	[90]
	hu	250 µm	7 days	Submerged	Model evaluation	[91]
	hu	250 µm	3 days	Submerged	Establishing a chemotherapy model	[92]
Breast (xenograft)	hu	200 µm	4 days	Submerged	Drug testing	[93]
Breast and prostate PDX models	hu	300 µm	2 weeks (breast), 1 week (prostate)	In chip, submerged, shear stress, perfusion	Cancer on chip platform for predicting drug response	[94]
Head and Neck Squamous Cell Carcinoma	hu	300 µm	5 days	Rotating platform	Model for evaluation of treatment response to radiation and chemotherapy	[95]

ALI Air–liquid interface, PDX Patient-derived xenograft, hu Human, ms mouse, sh sheep