Table 2. Crystallization conditions.
Method | Vapour diffusion, sitting drop |
Plate type | MRC 2-drop 96-well plate (Douglas Instruments) |
Temperature (K) | 277.15 |
Protein concentration (stock) (mg ml−1) | 13.7 |
Buffer composition of protein solution | 20 mM Tris pH 8.0 |
Composition of reservoir solution | A, 0.2 M ammonium formate pH 6.6, 20%(w/v) PEG 3350; B, 0.1 M potassium thiocyanate, 30%(w/v) PEG MME 2000 |
Volume and ratio of drop | 0.3 µl, 3:1 protein stock:reservoir solution |
Volume of reservoir (µl) | 100 |