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. 2023 May 25;79(Pt 6):545–555. doi: 10.1107/S205979832300325X

Table 2. Crystallization conditions.

Method Vapour diffusion, sitting drop
Plate type MRC 2-drop 96-well plate (Douglas Instruments)
Temperature (K) 277.15
Protein concentration (stock) (mg ml−1) 13.7
Buffer composition of protein solution 20 mM Tris pH 8.0
Composition of reservoir solution A, 0.2 M ammonium formate pH 6.6, 20%(w/v) PEG 3350; B, 0.1 M potassium thiocyanate, 30%(w/v) PEG MME 2000
Volume and ratio of drop 0.3 µl, 3:1 protein stock:reservoir solution
Volume of reservoir (µl) 100